If VLa and VLb light chain homology > 80%, design a VLc based on VLa
via computer aided design: Maintain VHaVLc’s affinity as close as that of
VHaVLa, allow VHbVLc to have reduced affinity compared to VHbVLb.
If VLa and VLb light chain homology < 80%, construct phage ScFV library:
VHs of the ScFV library are human germline VH library
VLs of the ScFV library are VLa and its mutants derived from error prone PCR
at less than 20% of mutation frequency.
Use computer aided design to separate the biochemical and biophysical features
of VHa and VHb in order to isolate AB from AA and BB at a higher efficiency.
Novel CNS Specific Antibody Platform
"Serial" Internalizing Antibody Platform
Unique feature of second strategy: Screening internalization caused by primary Ab.
Our method avoids internalization triggered by secondary antibody binding
Ab Studio Inc. is a start-up Biotech founded in Feb., 2017 in San Francisco bay area, USA. Founder and leading scientist have extensive expertise in the field of therapeutic antibody discovery, lead selection, engineering, humanization and optimization.
We look forward to developing novel therapeutic antibodies to target difficult to treat diseases via computer aided design.