Therapeutic Antibody Discovery Partnership


1. Monoclonal Antibody discovery service: hybridoma and phage display

2. Bispecific antibody discovery partnership

3. Internalization antibody discovery partnership

Workflow And Timeline For Antibody Discovery With Hybridoma Technology



Membrane Protein Immunization



WORK FLOW DESCRIPTION TIMELINE PRICE
Phase I
DNA preparation
DNA synthesis and plasmids construction 4 weeks $5,000
Phase II
Animal immunization(DNA immunization + cell immunization) and titer testing
Immunize the animals(n=4) for 3 times in 4 weeks(Cell be used in final boost ); Test anti-serum titers by ELISA 8-9 weeks $10,000
Phase III
Cell fusion and hybridoma growth on HAT medium and primary screening
Collect the spleen cells and fuse with myeloma cells to generate hybridoma cells in medium containing HAT; Primary screening 3-4 weeks $20,000
Phase IV
Subcloning and screening, expansion, and cryopreservation
Subcloning by limited dilution and Screening by ELISA; Select 10 to 20 candidate monoclones for expanding, cryopreservating and RNA later preserving 3-4 weeks $10,000 to $15,000
Phase V
Antibody sequencing
RNA extraction, 5'-RACE PCR, Sequencing(Fv region) of 10 to 20 leads 3 weeks $10,000 to $20,000
Phase VI
Monoclonal Ab, production and purification
Ab purification for 3 leads 3 weeks $10,000
Phase VII Customized design(developed from the very beginning) according to different usage To be determined To be determined

Our DNA immunization is applying IP free protocol



Soluble Protein Immunization



WORK FLOW DESCRIPTION TIMELINE PRICE
Phase I
Antigen preparation and screening assay development
Antigen preparation in mammalian cell expression system 6-8 weeks $10,000
Phase II
Animal immunization and titer testing
Immunize the animals(n=4) for 3 times in 6 weeks; Test anti-serum titers by ELISA 9-10 weeks $5,000
Phase III
Cell fusion and hybridoma growth on HAT medium and primary screening
Collect the spleen cells and fuse with myeloma cells to generate hybridoma cells in medium containing HAT; Primary screening 3-4 weeks $20,000
Phase IV
Subcloning and screening, expansion, and cryopreservation
Subcloning by limited dilution and Screening by ELISA; Select 10 to 20 candidate monoclones for expanding, cryopreservating and RNA later preserving 3-4 weeks $10,000 to $15,000
Phase V
Antibody sequencing
RNA extraction, 5'-RACE PCR, Sequencing(Fv region) of 10 to 20 leads 3 weeks $10,000 to $20,000
Phase VI
Monoclonal Ab, production and purification
Ab purification for 3 leads 3 weeks $10,000
Phase VII Customized design(developed from the very beginning) according to different usage To be determined To be determined

Phage Display


  1. Antigen specific ScFV phage library construction with immunized mouse B cells (if needed), 3-4 months, $30,000
  2. Panning with Ab Studio’s in house human naïve ScFV phage library or the new constructed antigen specific library, 1 month, $30,000 for 10 binding leads
  3. Analyze VH:VL interface naturality and developability of ScFV sequence, 1 month, $30,000
  4. Lead IgG production and characterization with assay developed by clients, 1 month, price TBD