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  • 01

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    Detailed sequence analysis and 3D structure modeling

  • 02

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    Identification of the best human germline sequence templates and, grafting the rodent or rabbit antibody CDR regions into the human antibody frameworks

  • 03

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    Identifying back-mutations and removing “hot spots” with developability issues

  • 04

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    High-throughput lead selection and evaluation

  • 05

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    Efficient lead preparation for research purpose in house (up to 20mg)and, CMC level proteinpreparation at the facility of our CMC partner (http://www.mab-venture.com/en/index.asp)

Antibody humanization and optimization workflow

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service workflow

Fv Modeling

Application of Schrodinger’s equation to build up one Fv homology model and one Fv hetero model

Generate an aggregation surface report

Generate a PTM analysis report (including N-glycosylation, deamidation, oxidation and protease site analysis based on the displaying status of the amino acid on the 3D structure surface)

Generate 3D PI prediction

Humanization and optimization design

Up to 5 humanized VH and 5 humanized VL versions will be designed respectively

PTMs will be removed if the antibody’s affinity and/or developability won’t be significantly affected

Changes of the 3D PI and aggregation potential will be marked during the design process, If a developability issue is observed on the bench, this Information will be applied to fix the developability problem of the humanization lead

The Predicted high risk T-cell epitope, B cell epitope, MHC-II epitope and antigenicity epitope will be recorded.

Clients will be informed about the potential complications to remove these epitopes

First round of lead selection and production

DNA synthesis, cloning and transient transfection

Affinity (ELISA) assay with expression medum

Aggregation potential and thermos-stability raw screening (ELISAs after heating) with expression medum

Production of the top 3 humanization leads and chimeric antibody (2mg each)

Non-specific binding assay (ELISA on Baculovirus) with in-house controls

Second round of final humanization lead selection

Affinity: Probelife (similar to Octect for kd detection) or a cell based assay (such as FACS, if other methods are not applicable)

Aggregation potential: DLS

Thermostability: DSF

Purity: CE

Price list, payment method and timeline

$30,000

GOLDEN DESIGN

  • No.01 + No.02
  • UPFRONT PAYMENT: $20,000
  • DESIGN FEE PAYMENT: $10,000
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GOLDEN DESIGN

$60,000

GOLDEN DESIGN+ PRELIMINARY SCREENING

  • No.01 + No.02 + No.03
  • UPFRONT PAYMENT: $20,000
  • PRELIMINARY SCREENING PAYMENT: $ 30,000
  • DESIGN FEE PAYMENT: $10,000

GOLDEN DESIGN+ PRELIMINARY SCREENING

$90,000

DIAMOND FULL HUMANIZATION SERVICE

  • No.01 + No.02 + No.03+ No.04
  • UPFRONT PAYMENT: $20,000
  • PRELIMINARY SCREENING PAYMENT: $ 30,000
  • DESIGN FEE PAYMENT: $10,000
  • SECONDARY ROUND SCREENING PAYMENT: $20,000
  • LEADING IGG PRODUCTION AND CHARACTERIZATION PAYMENT: $10,000

DIAMOND FULL HUMANIZATION SERVICE

Price list, payment method and time line Not only success based charge, but also service guarantee based charge!

The Humanization and optimization design starts after receiving a $20,000 upfront payment, A design report without sequence information will be sent to the client for approval within 2 weeks. Preliminary screening will start after the client approves the design and pays the $15,000 service fee. Preliminary screening data will be sent to clients in 6 weeks without second round of sequence optimization for affinity (~ 90% frequency) or 10 weeks with second round of sequence optimization for affinity (~ 10% frequency). After the data are received and approved by the client, a $35,000 protein production, purification, and second round screening fee needs to be sent to Ab Studio. After receiving the payment, Ab Studio will start the secondary round screening service, which will take 8 weeks without further sequence optimization for developability (~80% frequency) or 12 weeks with further sequence optimization for developability (~20% frequency). The data from the second round of screening will be sent to the client for selecting the final lead sequence. Once the client informs Ab Studio the final lead and sends us $10,000 lead production fee as well as $10,000 remaining design fee, Ab Studio will purify 10mg of the final lead antibody protein and send it to client along with the remaining purified antibodies from first round of screening as well as the full antibody humanization/ optimization report (containing all designed sequences).

Significant discounts will be applied for start-ups and/or multiple projects!
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