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01

Partnerships for bispecific antibody discovery

Apply “knob into hole” and common light chain technologies for bispecific antibody development

Use Ab Studio’s unique methodology to screen common light chains

Apply computer-aided design to engineer the two arms of a bi-specifc antibody for optimal purification efficacy

02

Partnerships for internalization antibody discovery

Apply Ab Studio’s unique methodology to screen hybridoma clones secreting cancer-specifc internalization antibodies or phages displaying internalization antibodies

Workflow and Timeline for Antibody Discovery with Hybridoma Technology

Membrane Protein Immunization

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  • WORK FLOW
  • DESCRIPTION
  • TIMELINE
  • WORK FLOWPhase VII
  • DESCRIPTIONCustomized design(developed from the very beginning) according to different usage
  • TIMELINETo be determined
  • WORK FLOWPhase VI Monoclonal Ab, production and purification
  • DESCRIPTIONAb purification for 3 leads
  • TIMELINE3 weeks
  • WORK FLOWPhase V Antibody sequencing
  • DESCRIPTIONRNA extraction, 5'-RACE PCR, Sequencing(Fv region) of 10 to 20 leads
  • TIMELINE3 weeks
  • WORK FLOWPhase IV Subcloning and screening, expansion, and cryopreservation
  • DESCRIPTIONSubcloning by limited dilution and Screening by ELISA; Select 10 to 20 candidate monoclones for expanding, cryopreservating and RNA later preserving
  • TIMELINE3-4 weeks
  • WORK FLOWPhase III Cell fusion and hybridoma growth on HAT medium and primary screening
  • DESCRIPTIONCollect the spleen cells and fuse with myeloma cells to generate hybridoma cells in medium containing HAT; Primary screening
  • TIMELINE3-4 weeks
  • WORK FLOWPhase II Animal immunization(DNA immunization + cell
  • DESCRIPTIONImmunize the animals(n=4) for 3 times in 4 weeks(Cell be used in final boost ); Test anti-serum titers by ELISA
  • TIMELINE8-9 weeks
  • WORK FLOWPhase I DNA preparation
  • DESCRIPTIONDNA synthesis and plasmids construction
  • TIMELINE4 weeks

Our DNA Immunization Applies IP-free Protocol

Soluble Protein Immunization

  • Title
  • DESCRIPTION
  • TIMELINE
  • TitlePhase VII
  • DESCRIPTIONCustomized design(developed from the very beginning) according to different usage
  • TIMELINETo be determined
  • TitlePhase VI Monoclonal Ab, production and purification
  • DESCRIPTIONAb purification for 3 leads
  • TIMELINE3 weeks
  • TitlePhase V Antibody sequencing
  • DESCRIPTIONRNA extraction, 5'-RACE PCR, sequencing(Fv region) of 10 to 20 leads
  • TIMELINE3 weeks
  • TitlePhase IV Subcloning and screening, expansion, and cryopreservation
  • DESCRIPTIONSubcloning by limited dilution and Screening by ELISA; Select 10 to 20 candidate monoclones for expanding, cryopreservating and RNA later preserving
  • TIMELINE3-4 weeks
  • TitlePhase III Cell fusion and hybridoma growth on HAT medium and primary screening
  • DESCRIPTIONCollect the spleen cells and fuse with myeloma cells to generate hybridoma cells in medium containing HAT; Primary screening
  • TIMELINE3-4 weeks
  • TitlePhase II Animal immunization(DNA immunization + cell immunization) and titer testing
  • DESCRIPTIONImmunize the animals(n=4) 3 times within 6 weeks; determine anti-serum titers by ELISA
  • TIMELINE8-9 weeks
  • TitlePhase I Antigen preparation and screening assay development
  • DESCRIPTIONAntigen preparation in a mammalian cell expression system
  • TIMELINE6-8 weeks

The Phage Display

Antigen specific ScFV phage library construction with immunized mouse B cells (if needed), 3-4 months, price TBD

Panning with Ab Studio’s in house human naïve ScFV phage library or the newly constructed antigen specific library, 1 month, price TBD for 10 binding leads

Analyze the VH:VL interface naturality and developability the of ScFV sequence, 1 month, price TBD

Lead IgG production and characterization with aclient-developed assay, 1 month, price TBD

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